Dataset Information

Comparative Proteomics Reveals Physiological and Molecular Mechanisms Underlying Sour Jujube Resistance to Jujube Witches' Broom Disease

ABSTRACT: Jujube is an important medicinal plant and economic crop in China. However, long-term infestation by jujube witches’ broom disease has led to significant yield losses. In this study, TMT-based quantitative proteomics was employed to investigate the molecular mechanisms underlying disease resistance. Our results revealed that differentially expressed proteins were predominantly localized in the cytoplasm and chloroplasts, which function synergistically to maintain cellular homeostasis. Chloroplasts generate ATP and NADPH through photosynthesis, supplying energy and reducing power for various metabolic activities, while cytoplasmic pathways such as glycolysis and the tricarboxylic acid cycle provide essential precursor substances for chloroplast-based photosynthetic processes. These findings demonstrate the coordinated regulation of energy metabolism at the subcellular level, offering a theoretical foundation for the identification and screening of disease-resistant proteins.

ORGANISM(S): Ziziphus Jujuba Var. Spinosa

SUBMITTER: Donglai Ma

PROVIDER: PXD074213 | iProX | Sat Feb 07 00:00:00 GMT 2026

REPOSITORIES: iProX

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Integrated carbon metabolism-transcriptomics-proteomics reveals physiological and molecular mechanisms underlying sour jujube resistance to witches' broom disease.

Wang Chaohui C Yan Yuping Y Lu Zikang Z Li Hongjie H Sun Huigai H Pei Lin L Zhang Ye Y Fu Xiangrui X Hou Jiahao J He Pei P Guo Hui H Song Yongxing Y Gu Xian X Han Xiaowei X Ma Donglai D

BMC plant biology 20260611

Phytoplasma-mediated witches' broom disease (WBD) causes abnormal growth and inhibits fruit formation in sour jujube trees, causing significant economic damage. Research on the pathogenic mechanisms of WBD and the disease resistance mechanisms of sour jujube tree remains poorly understood. In this study, these mechanisms were investigated using healthy resistant jujube branches that had been grafted onto WBD-infected trees and grown for seven years. Leaves were collected from both healthy resist ...[more]

PMID: 42277659

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Project description:Exposure to hypoxia disrupts energy metabolism and induces inflammation. However, the pathways and mechanisms underlying energy metabolism disorders caused by hypoxic conditions remain unclear. In this study, we constructed a hypoxic animal model and applied transcriptomic and non-targeted metabolomics techniques to further investigate the pathways and mechanisms of hypoxia exposure that disrupt energy metabolism. Transcriptome results showed that 3007 genes were significantly differentially expressed under hypoxic exposure, and Gene Ontology (GO) annotation analysis and Kyoto Encyclopaedia of Genes and Genomes (KEGG) enrichment analysis showed that the differentially expressed genes (DEGs) were mainly involved in energy metabolism and were significantly enriched in the tricarboxylic acid (TCA) cycle and oxidative phosphorylation (OXPHOS) pathway. Differential genes in the TCA cycle (IDH3A, SUCLA2, and MDH2) and OXPHOS pathway (NDUFA3, NDUFS7, UQCRC1, CYC1, and UQCRFS1) were validated using mRNA and protein expression, and the results showed downregulation. The results of non-targeted metabolomics showed that 365 significant differential metabolites were identified under plateau hypoxia stress. KEGG enrichment analysis showed that the differential metabolites were mainly enriched in metabolic processes, such as energy metabolism, nucleotide metabolism, and amino acid metabolism. Hypoxia exposure disrupted the TCA cycle and reduced the synthesis of amino acids and nucleotides by decreasing the concentrations of cis-aconitate, α-ketoglutarate, NADH, NADPH, most amino acids, purines, and pyrimidines. Bioinformatics analysis was used to identify inflammatory genes related to hypoxia exposure, and some inflammatory genes were selected for verification. We found that the mRNA and protein expression levels of IL1B, IL12B, S100A8, and S100A9 in kidney tissues were upregulated under hypoxic exposure. Our results suggest that hypoxia exposure inhibits the TCA cycle and OXPHOS signalling pathway by inhibiting IDH3A, SUCLA2, MDH2, NDUFFA3, NDUFS7, UQCRC1, CYC1, and UQCRFS1, thereby suppressing energy metabolism, inducing amino acid and nucleotide deficiency, and promoting inflammation, ultimately leading to kidney damage.

Dataset Information

Comparative Proteomics Reveals Physiological and Molecular Mechanisms Underlying Sour Jujube Resistance to Jujube Witches' Broom Disease

Publications

Integrated carbon metabolism-transcriptomics-proteomics reveals physiological and molecular mechanisms underlying sour jujube resistance to witches' broom disease.

Similar Datasets

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