Proteomics

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Tumour-associated high endothelial venules drive portal-specific immune evasion in lymph nodes via ALOX12

ABSTRACT: This project aims to characterize the endothelial cell proteome labeled by tumor-derived secreted Sema3C in metastatic lymph nodes using an in vivo TurboID-based proximity labeling strategy. EO771 breast cancer cells overexpressing either a secreted p65-Sema3C–TurboID fusion protein or TurboID alone were orthotopically implanted into C57BL/6 mice. Following tumor growth and lymph node colonization, systemic biotin administration enabled proximity-dependent biotinylation of proteins in neighboring host endothelial cells. Primary high endothelial cells (HECs) were isolated from metastatic lymph nodes, and biotinylated proteins were enriched and analyzed by high-resolution data-independent acquisition (DIA) mass spectrometry. This dataset provides a resource for identifying Sema3C-associated endothelial proteins in the metastatic tumor microenvironment in vivo.

ORGANISM(S): Mus Musculus

SUBMITTER: Shicheng Su  

PROVIDER: PXD074282 | iProX | Tue Feb 10 00:00:00 GMT 2026

REPOSITORIES: iProX

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High endothelial venules (HEVs) provide another portal for tumour metastasis. However, whether HEVs and other blood vessels exert different effects on tumour escape remains unknown. Here we show that tumour involvement in HEVs is an independent prognostic marker for patients with lymph node (LN)-positive female breast cancer. Tumour cells that spread via HEVs are less immunogenic and more capable of establishing distant metastases than those that spread through non-HEV blood vessels. Mechanistic  ...[more]

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