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TurboID-based proximity labeling to identify BRD4 and MEF2C co-interacted proteins

ABSTRACT: Our studies suggest that BRD4 plays a critical role in muscle fiber-type regulation, yet the molecular mechanisms by which BRD4 cooperates with MEF2 to control muscle gene expression remain incompletely understood. To identify potential bridging factors that facilitate BRD4-MEF2 interaction, unbiased TurboID-based proximity labeling was employed to map BRD4- and MEF2-associated proteins. Comparative analysis of the BRD4- and MEF2C-TurboID proximity interactomes revealed that CHD4, a core component of the NuRD chromatin-remodeling complex, was identified as a prominent candidate. Functional protein association networks analysis indicated that these shared proteins are related to chromatin organization and transcriptional regulation.

ORGANISM(S): Homo Sapiens

SUBMITTER: Lei Fang

PROVIDER: PXD075119 | iProX | Mon Mar 02 00:00:00 GMT 2026

REPOSITORIES: iProX

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