Project description:For a study comparing small extracellular vesicles (sEVs) from WI-38 and A549 cells. The proteome of A549 sEVs were obtained to find proteins that could distinguish WI-38-derived sEVs from A549-derived sEVs. These protein markers were used as proof-of-principle biomarkers for a FRET-based sEV biomarker assay.
Project description:This project aims to study the molecular signature of glucocorticoid treatment on A549 human lung adenocarcinoma cells on transcriptome and proteome levels. Glucocorticoid treatment was previously identified to induce formation of cytoplasmic Processing-bodies (P-bodies) in epithelial cell lines, which results in the recruitment of mRNA into P-bodies for storage and/or degradation.