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HflX mediates rifampicin resistance in Brucella by downregulating the expression of RNA polymerase-associated genes

ABSTRACT: To further investigate molecular changes associated with HflX knock during rifampicin exposure, we performed label-free quantitative proteomic analysis. The WT and ΔhflX strains were cultured for three generations in the presence of 1/2 MIC rifampicin (1 μg/mL) and harvested during the logarithmic growth phase, with at least three biological replicates per group. DIA-based proteomic analysis quantified protein abundance and identified differentially expressed proteins (DEPs) (Figure 5A). Bioinformatic analyses were then conducted to evaluate enriched pathways and co-expression networks, aiming to elucidate the regulatory role of HflX in Brucella.

ORGANISM(S): Brucella Abortus 2308

SUBMITTER: Yuefeng Chu

PROVIDER: PXD075846 | iProX | Thu Mar 19 00:00:00 GMT 2026

REPOSITORIES: iProX

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HflX mediates rifampicin resistance in Brucella by downregulating the expression of RNA polymerase-associated genes.

Geng Hao H Qi Mengzhu M Su Mengru M Zhi Feijie F Chu Yuefeng Y

Frontiers in microbiology 20260415

Brucella species (Brucella spp.) are facultative intracellular zoonotic pathogens responsible for brucellosis, a disease causing substantial global public health and economic burdens. Rifampicin remains a first-line therapeutic agent, but the molecular mechanisms underlying rifampicin resistance in Brucella remain poorly defined, especially the contribution of ribosome-associated regulatory proteins. HflX is a conserved ribosome-binding GTPase involved in ribosomal quality control ...[more]

PMID: 42063517

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