Proteomics

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Identification of Lysine-Cysteine Crosslinking Sites in Homobifunctional NHS-Ester Crosslinkers


ABSTRACT: NHS esters are commonly used reactive groups in crosslinking chemistry. We first demonstrated that NHS esters can react with the side-chain thiol group of cysteine and observed lysine-cysteine (K-C) crosslinks when using homobifunctional crosslinkers. However, in conventional crosslinking workflows, K-C crosslinks are difficult to identify. To address this, we explored factors affecting the stability of K-C crosslinks within the experimental pipeline. By employing an optimized workflow, we were able to consistently identify a greater number of K-C crosslinking sites while maintaining the detection of lysine-lysine (K-K) crosslinks.

ORGANISM(S): Homo Sapiens Bos Taurus

SUBMITTER: Xiaoming Zhang  

PROVIDER: PXD075905 | iProX | Thu Mar 19 00:00:00 GMT 2026

REPOSITORIES: iProX

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