Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Slc44a2 deficiency unveils an IFN-I–dependent feedback control of pDC egress

ABSTRACT: This project aims to characterize the proteomic landscape of bone marrow-derived plasmacytoid dendritic cells (pDCs) from wild-type (WT) and Slc44a2-deficient (cKO) mice.

ORGANISM(S): Mus Musculus

SUBMITTER: Ligong Chen

PROVIDER: PXD076360 | iProX | Tue Mar 31 00:00:00 BST 2026

REPOSITORIES: iProX

ACCESS DATA

Json Xml

Similar Datasets

The role of CD11c in dendritic cells

Project description:We cultured bone marrow derived dendritic cells from WT and CD11c KO mice. Then, a group of bone marrow dendritic cells were stimulated with LPS overnight. We obtained bone marrow derived dendritic cells with or without LPS stimulation and analyzed proteomics profiles.

2024-01-26 | PXD048907 |

RhoA ko BMDC - Control of Homeostasis and Dendritic Cell Survival by the GTPase RhoA

Project description:Differential protein expression between RhoA ko and wt bone marrow-derived dendritic cells

2015-10-01 | PXD001636 | Pride

Tec kinase deficiency in macrophages

Project description:Previous work has shown conflicting roles for Tec family kinases in regulation of Toll-like receptor (TLR)-dependent signalling in myeloid cells. In the present study, we performed a detailed investigation of the role of Btk and Tec kinases in regulating TLR signalling in several types of primary murine macrophages. We demonstrate that primary resident peritoneal macrophages deficient for Btk and Tec secrete less pro-inflammatory cytokines in response to TLR stimulation than wild type cells. In contrast, we found that bone marrow-derived and thioglycollate-elicited peritoneal macrophages deficient for Btk and Tec secrete more pro-inflammatory cytokines than wild type cells. We then compared the phosphoproteome regulated by Tec kinases and lipopolysaccharide in primary peritoneal and bone marrow derived macrophages. From this analysis we determined that Tec kinases regulate different signalling programs in these cell types. In additional studies using bone marrow-derived macrophages, we find that Tec and Btk promote phosphorylation events necessary for immunoreceptor-mediated inhibition of TLR signalling. Taken together, our results are consistent with a model where Tec kinases (Btk, Tec, Bmx) are required for TLR-dependent signalling in many types of myeloid cells. However, our data also support a cell type-specific TLR-inhibitory role for Btk and Tec that is mediated by immunoreceptor activation and signalling via PI3K.

2018-10-24 | PXD002031 | Pride

p16INK4a-deficiency promotes macrophages from classical to alternative activation

Project description:murine p16ink4a deficient (p16ko) and control (p16wt) bone marrow cells were either differentiated with normal LCM-supplemented differentiation medium to obtain bone marrow derived macrophages (BMDM) or supplemented with Interleukin 4 during differeniation to obtain M2 polarized p16wt and p16ko BMDM.

2011-07-01 | E-MEXP-3216 | biostudies-arrayexpress

Effect of LPS or IFN-beta activation on gene expression of bone marrow-derived dendritic cells

Project description:To examine the changes in genes encoding proteins of metabolic pathways induced by LPS or IFN-beta in bone marrow-derived dendritic cells.

2022-05-29 | GSE204955 | GEO

Control of RelB during dendritic cell activation integrates canonical and non-canonical NFkB pathways

Project description:Dendritic cells (DC) play central roles in coordinating appropriate immune responses to pathogens. In the present study, the genes regulated by NFkB activators, RelB and cRel, in PAMP-induced bone marrow dendritic cells (BMDCs) activation was examined. Total RNA extracted from WT, relb-/-, crel-/-relb-/- bone marrow derived dendritic cells (BMDCs) were subjected to stimulation with CpG or Pam3CSK4.

2012-10-01 | E-GEOD-34990 | biostudies-arrayexpress

Expression in IFN-gamma treated control and Atg5-/- macrophages

Project description:Type-I (α/β) and -II (γ) interferons (IFN), through an incompletely understood combination of redundant and unique mechanisms, are essential for host resistance to viral infection. We report a requirement for the Atg5-Atg12/Atg16L1 autophagosome elongation complex in IFNγ-mediated control of murine norovirus in macrophages. We use microarrays to compare transcriptional changes induced in control and Atg5 deficient macrophages by IFNγ treatment. Bone marrow derived macrophages were generated from Atg5flox/flox and Atg5flox/flox+LysM cre mice and treated with media alone or 100U/ml of IFNγ for 14 hrs. RNA was extracted using Trizol (Invitrogen) and 10 µg of RNA hybridized to Affymetrix microarrays.

2012-01-05 | E-GEOD-34863 | biostudies-arrayexpress

RNA-Seq Analysis of FACS-sorted GFP-expressing and skeletal stem cells (SSC) from the bone marrow of mice with S1P ablation in the Osterix (Osx)-lineage (Cko)

Project description:Comparison of the transcriptome of GFP-expressing cells from the bone marrow compartment of Cko mice with the transcriptome of skeletal stem cells (SSC) from the bone marrow compartment of Cko and WT mice (homozygous floxed S1P mice).

2020-06-04 | GSE146304 | GEO

Effect of PGC-1beta deficiency on gene expression of bone marrow-derived dendritic cells

Project description:To determine the changes in gene expression regulated by PGC-1beta, we knocked down PGC-1beta in bone marrow-derived dendritic cells by shRNA.

2022-05-29 | GSE204954 | GEO