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Transcriptome and proteome elucidate the mechanism of spermidine-eIF5Ahyp axis targeted TUFM in regulating autophagy in porcine granulosa cells

ABSTRACT: Autophagy is a conserved pathway essential for granulosa cell homeostasis and follicular development. Spermidine, the sole substrate for eIF5A hypusination (eIF5Ahyp), promotes autophagy and exerts diverse protective effects, including antioxidant and anti-aging activities. This study demonstrates that the spermidine-eIF5Ahyp axis influences autophagy in porcine ovarian granulosa cells by regulating the expression of the downstream protein TUFM. These findings provide mechanistic insights into autophagic regulation in reproductive cells and highlight potential targets for enhancing mammalian fertility.

ORGANISM(S): Sus Scrofa

SUBMITTER: Bo Kang

PROVIDER: PXD077497 | iProX | Wed Apr 22 00:00:00 BST 2026

REPOSITORIES: iProX

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