Simple Workflow for Integrated and Fast Tissue-preparation for microgram-level FF and FFPE Tissues Proteomics Analysis

ABSTRACT: We present Simple Workflow for Integrated and Fast Tissue-preparation (SWIFT) workflows, enabling complete processing of fresh-frozen (FF) and formalin-fixed, paraffin-embedded (FFPE) tissues in one- or two-step formats, while maintaining deep proteome coverage with good repeatability. For FF tissues, an incubation process integrating lysis, reduction, alkylation, and digestion can generate peptide samples directly from tissues in ≤ 1.5 h. For FFPE tissues, simultaneous deparaffinization, rehydration, and de-crosslinking was achieved within 0.5 h, followed by one-step pretreatment to obtain peptides.

ORGANISM(S): Homo Sapiens Mus Musculus

SUBMITTER: Yun Yang  

PROVIDER: PXD077679 | iProX | Mon Apr 27 00:00:00 BST 2026

REPOSITORIES: iProX