Dataset Information

Proteomic analysis of cadmium immobilization by bacterial consortia of Achromobacter insuavis SL8 and Enterobacter cancerogenus SL12

ABSTRACT: Strains Achromobacter insuavis and Enterobacter cancerogenus, as well as their co-culture, were grown in LB medium with/without 100 mg/L Cd²⁺ for 48 h. For label‑free proteomics, cells were lysed in urea/thiourea/CHAPS buffer, homogenized, sonicated, and centrifuged. The protein precipitate was analyzed by LC-MS/MS (Agilent 1290‑TripleTOF 5600).

ORGANISM(S): Achromobacter Insuavis Enterobacter Cancerogenus

SUBMITTER: Xin Li

PROVIDER: PXD078447 | iProX | Fri May 15 00:00:00 GMT+01:00 2026

REPOSITORIES: iProX

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A study on cadmium immobilization by bacterial consortia: Achromobacter insuavis SL8 and Enterobacter cancerogenus SL12.

Tao Yu Y Ji Shengying S Zhou Chi C Peng Di D Li Xin X

Microbiology spectrum 20260617 7

Microbial bioremediation represents one of the most environmentally sustainable strategies for mitigating cadmium (Cd) pollution. This study employed an integrated approach combining spectroscopy, metabolomics, and proteomics to elucidate the molecular mechanisms underlying Cd detoxification in Achromobacter insuavis SL8 and Enterobacter cancerogenus SL12. The results demonstrated that both strains exhibit high Cd tolerance, with minimum inhibitory concentrations (MICs) of 600 mg/L ...[more]

PMID: 42307251

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Project description:Purpose: Identify gene expression changes in whole brains of newborn pups whose mothers were exposed to Cd during pregnancy (50ppm CdCl2) compared to unexposed controls (0ppm CdCl2). Methods: 5- to 7-week-old C57BL/6J (‘B’) and CAST/EiJ (‘C’) females (the F0 generation) were exposed through their drinking water to 0 ppm or 50 ppm Cd in the form of CdCl2 (Sigma-Aldrich, catalog number 202908) for a period of 5 weeks, then mated with a male of the opposite strain. Cd exposure continued throughout pregnancy and was discontinued at litter birth. A hybrid mating scheme (the F1 generation) was employed to enable analyses of allele-specific gene expression and DNA methylation for a separate study (Hudson & Cowley, manuscript in preparation). The genotypes of F1 mice are referred to as ‘BxC’ (B mother x C father). F1 animals were sacrificed and dissected within 24 hours of birth; sample sizes can be found in supplementary tables associated with the publication. F1 mice were dissected within 24 hours of birth. All animal work was approved by the North Carolina State University (NCSU) Institutional Animal Care and Use Committee, under protocol 16-045-B. All experiments were conducted in accordance with the Guiding Principles in the Use of Animals in Toxicology. Frozen whole brains obtained from four 0 ppm and four 50 ppm newborn female BxC pups representing four litters per exposure group were pulverized using a hammer and liquid nitrogen. 7-10 mg of tissue was then used to extract DNA and RNA simultaneously using the AllPrep DNA/RNA/miRNA kit (Qiagen, 80204). RNA was extracted using the recommended protocol for fatty tissues. RNA and DNA were quantified using a Nanodrop 2000. RNA purity and size integrity were determined at the NCSU Genomic Sciences Laboratory (GSL) using an Agilent 2100 Bioanalyzer with an RNA 6000 Nano Chip (Agilent Technologies). Results: We identified 15 significantly differentially expressed genes (DEGs) that aligned to both the B and C genomes. Conclusions: These 15 DEGs (and subsequent confirmatory qRT-PCRs on the 8 samples sequenced here plus an additional Cd dose and samples) suggest altered onset of myelination throughout the whole brain, as well as aberrant Hox gene expression due to maternal Cd exposure.

Dataset Information

Proteomic analysis of cadmium immobilization by bacterial consortia of Achromobacter insuavis SL8 and Enterobacter cancerogenus SL12

Publications

A study on cadmium immobilization by bacterial consortia: <i>Achromobacter insuavis</i> SL8 and <i>Enterobacter cancerogenus</i> SL12.

Similar Datasets

OmicsDI is part of the ELIXIR infrastructure

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Dataset Information

Proteomic analysis of cadmium immobilization by bacterial consortia of Achromobacter insuavis SL8 and Enterobacter cancerogenus SL12

Publications

A study on cadmium immobilization by bacterial consortia: &lt;i&gt;Achromobacter insuavis&lt;/i&gt; SL8 and &lt;i&gt;Enterobacter cancerogenus&lt;/i&gt; SL12.

Similar Datasets

OmicsDI is part of the ELIXIR infrastructure

Tweets

A study on cadmium immobilization by bacterial consortia: <i>Achromobacter insuavis</i> SL8 and <i>Enterobacter cancerogenus</i> SL12.