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ILC2-intrinsic IL-33 promotes ILC2 activation and exacerbates allergic lung 1 inflammation

ABSTRACT: Mass spectrometry-based proteomics serves as an invaluable tool for pinpointing critical biomarkers and elucidating cellular functions and regulatory pathways. However, the proteomic changes in ILC2s during activation remain unexplored. To characterize the dynamic proteomic profiles of lung-resident ILC2s under physiological and inflammatory conditions, we performed high-resolution liquid chromatography-tandem mass spectrometry (LC-MS/MS) to systematically compare the molecular signatures of FACS-purified pulmonary ILC2 populations (Live CD45+CD90.2+Lineage- ST2+) isolated from two experimental groups: 1) steady-state control mice and 2) papain-challenged mice exhibiting acute airway inflammation (Fig. 1a and Supplementary Fig. 1a). Papain-induced lung inflammation was chosen because papain is a classical protease allergen triggering the release of pro-inflammatory factors including IL-33 to develop ILC2-derived lung inflammation.

ORGANISM(S): Mus Musculus

SUBMITTER: Rong Zeng

PROVIDER: PXD078462 | iProX | Sat May 16 00:00:00 GMT+01:00 2026

REPOSITORIES: iProX

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