Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Hos2-mediated KatG1 decrotonylation orchestrates ROS scavenging in rice blast infection

ABSTRACT: In plant–pathogen interaction, plants trigger reactive oxygen species (ROS) bursts to defend against pathogen invasion, while pathogens evolved antioxidant systems to scavenge ROS for infection. Our study reveals a novel regulatory mechanism of ROS adaptation and scavenging in the rice blast fungus Magnaporthe oryzae. We found that KatG1, a bifunctional catalase-peroxidase, acts as a ROS-responsive regulatory enzyme. During infection, the deacetylase Hos2 mediates the decrotonylation of KatG1 at K422, initiating the ROS clearance process. Decrotonylated KatG1 depolymerizes into monomers, interacts with the Hsp70 protein Ssb1, and translocates to the nucleus. Nuclear KatG1 promotes the dissociation of the Atf1-Tup1 complex, activating the expression of Atf1-mediated oxidoreductase genes and enhancing fungal infection. Moreover, we identified a small molecule, orcinol gentiobioside (OGB), that targets KatG1, inhibiting M. oryzae invasion. This study elucidates a new regulatory mechanism of ROS metabolism and stress adaptation in plant fungal infections and provides a potential target for fungicide development to control rice blast disease

ORGANISM(S): Pyricularia Oryzae 70-15

SUBMITTER: Xiaolin Chen

PROVIDER: PXD078794 | iProX | Sat May 23 00:00:00 BST 2026

REPOSITORIES: iProX

ACCESS DATA

Json Xml

Similar Datasets

Temporal gene expression analyisis from rice root (cv. Nipponbare) infected with Magnaporthe oryzae strain Guy11

Project description:Magnaporthe oryzae causes rice blast, the most devastating foliar fungal disease of cultivated rice. During disease development the fungus simultaneously maintains both biotrophic and necrotrophic growth corresponding to a hemi-biotrophic life style. The ability of M. oryzae to also colonize roots and subsequently develop blast symptoms on aerial tissue has been recognized. The fungal root infection strategy and the respective host responses are currently unknown. Global temporal expression analysis suggested a purely biotrophic infection process reflected by the rapid induction of defense response-associated genes at the early stage of root invasion and subsequent repression coinciding with the onset of intracellular fungal growth. The same group of down-regulated defense genes was increasingly induced upon leaf infection by M. oryzae where symptom development occurs shortly post tissue penetration. Our molecular analysis therefore demonstrates the existence of fundamentally different tissue-specific fungal infection strategies and provides the basis for enhancing our understanding of the pathogen life style.

2009-10-02 | GSE18361 | GEO

Transcription profiling of rice cv. Nipponbare roots infected with Magnaporthe oryzae strain Guy11

2009-10-09 | E-GEOD-18361 | biostudies-arrayexpress

Large-scale rewiring of innate immunity circuitry and microRNA regulation during initial rice blast infection [smallRNA-Seq]

Project description:Rice blast is a recurrent fungal disease, and resistance to fungal infection is a complex trait. Therefore, a comprehensive examination of rice transcriptome and its variation during fungal infection is necessary to understand the complex gene regulatory networks. In this study, adopting Next-Generation Sequencing we profiled the transcriptomes and microRNAomes of rice varieties, one susceptible and the other resistant to M. oryzae, at multiple time points during the fungal infection.

2016-05-01 | GSE77332 | GEO

Large-scale rewiring of innate immunity circuitry and microRNA regulation during initial rice blast infection [RNA-Seq]

2016-05-01 | GSE77331 | GEO

The phosphorylation landscape of infection-related development by the rice blast fungus

Project description:Many of the world’s most devastating crop diseases are caused by fungal pathogens which elaborate specialized infection structures to invade plant tissue. Here we present a quantitative mass spectrometry-based phosphoproteomic analysis of infection-related development by the rice blast fungus Magnaporthe oryzae, which threatens global food security. We mapped 8,005 phosphosites on 2,062 fungal proteins, revealing major re-wiring of phosphorylation-based signaling cascades during fungal infection. Comparingme phosphosite conservation across 41 fungal species reveals phosphorylation signatures specifically associated with biotrophic and hemibiotrophic fungal infection. We then used parallel reaction monitoring to identify phosphoproteins directly regulated by the Pmk1 MAP kinase that controls plant infection by M. oryzae. We define 33 substrates of Pmk1 and show that Pmk1-dependent phosphorylation of a newly identified regulator, Vts1, is required for rice blast disease. Defining the phosphorylation landscape of infection therefore identifies potential therapeutic interventions for control of plant diseases.

2024-04-03 | PXD044830 | Pride

MAP kinase-dependent cell-to-cell movement drives plant tissue invasion by the rice blast fungus

Project description:We created a mutant in the MAP kinase-encoding Pmk1 gene of the rice blast fungus Magnaporthe oryzae (pmk1AS) that renders the gene sensitive to inhibition by 1NA-PP1. Fungal gene expression was compared during infection of rice sheath by the M. oryzae pmk1AS mutant in the presence and absence of 1NA-PP1.

2018-03-01 | GSE106845 | GEO

Positive roles for Osa-miR7695 in regulating defense responses against the rice blast fungus

Project description:MicroRNAs (miRNAs) are small non-coding RNAs that function as post-transcriptional regulators of gene expression in eukaryotes. In rice, miR7695 targets an alternatively spliced transcript of natural resistance-associated macrophage protein 6 (OsNramp6) encoding an iron transporter whose expression is regulated by infection with the rice blast fungus Magnaporthe oryzae. Rice plants grown under high iron supply showed blast resistance, which supports that iron is a factor in controlling blast resistance by still unknown mechanisms. Here, iron accumulated near M. oryzae appressoria, the sites of pathogen entry, and in cells surrounding infected regions of the rice leaf. Activation-tagged MIR7695 rice plants (MIR7695-Ac) exhibited enhanced resistance to M. oryzae infection. RNA-seq analysis revealed that blast resistance in MIR7695-Ac plants was associated with strong induction of defense-related genes, including pathogenesis-related and diterpenoid biosynthetic genes. Levels of phytoalexins during pathogen infection were higher in MIR7695-Ac than wild-type plants. Early phytoalexin biosynthetic genes, OsCPS2 and OsCSP4, were highly upregulated in rice plants grown under high iron supply. Our data indicate that miR7695 positively regulates rice immunity while establishing links between defense and iron signaling in rice. MiR7695-mediated regulation of OsNramp6 has great potential for the development of strategies to control rice blast disease.

2020-01-08 | GSE122258 | GEO

SIRT5-mediated lysine desuccinylation regulates oxidative stress adaptation for intracellular infection of Magnaporthe oryzae

Project description:Plant-pathogenic fungi have developed kinds of detoxification strategies to suppress host reactive oxygen species (ROS) for colonization, but how this process is elaborately regulated has not been well revealed. Here we show that the SIRT5-mediated protein desuccinylation acts as a master regulator to regulate ROS detoxification in the rice blast fungus Magnaporthe oryzae. The sirtuin protein SIRT5 was identified as a desuccinylase in M. oryzae, deletion of which resulted in increased sensitivity to oxidative stress. Further analysis found that Sirt5 is important for fungal virulence and adaptation to host oxidative stress. Quantitative proteomics analysis under oxidative stress identified a large number of SIRT5-mediated succinylated proteins, most of which were mitochondrial proteins involved in oxidative phosphorylation, TCA cycle, fatty acid oxidation, etc. Many succinylated proteins were enzymes involved in different ROS de-toxification processes. Disruption of SIRT5 resulted in hypersuccinylation of detoxification-related enzymes, and significant reduction of NADPH/NADP+ and GSH/GSSG ratios, disrupting redox balance and impeding the expansion of invasive hyphae in the host. Interestingly, we proved that SIRT5 can desuccinylate thioredoxin Trx2 and glutathione peroxidase Hyr1 to activate its enzyme activity, probably by affecting its proper folding. Further analysis indicated that the succinylation sites of Trx2 (K144) and Hyr1 (K101, K127) were important for their function in ROS detoxification and virulence. Altogether, this work demonstrates a novel regulatory mechanism of SIRT5-mediated desuccinylation in detoxifying host ROS during M. oryzae infection.

2024-03-06 | PXD044696 | Pride

Rice Gene Expression During Biotrophic Invasion by Magnaporthe oryzae (Agilent Array G4138A)

Project description:The hemibiotrophic fungus Magnaporthe oryzae produces specialized biotrophic invasive hyphae (IH) that alter membrane structure and defense responses in invaded rice cells. IH successively invade live neighbor cells, apparently through plasmodesmata. Understanding fungal and rice genes that contribute to biotrophic invasion has been a challenge because so few plant cells have encountered IH at the earliest infection stages. Using a rice sheath inoculation method, we successfully enriched for infected tissue RNA that contained ~20% fungal RNA at a point when most IH were still growing in first-invaded rice cells. The RNAs were analyzed using the whole-genome M. oryzae oligoarray and a rice oligoarray. Rice genes that were induced >50-fold during infection were enriched for genes involved in transferring information from sensors to cellular responses. Fungal genes that were induced >50-fold in IH included the PWL2 avirulence gene and genes encoding hypothetical secreted proteins. The IH-specific secreted proteins are candidate effectors, proteins that the fungus secretes into live host cells to control cellular processes. Gene knock-out analyses of three putative effector genes failed to show major effects on pathogenicity. Details of the blast interaction transcriptome will provide insights on the mechanisms of biotrophic plant disease. Keywords: Disease state analysis

2009-04-08 | GSE8518 | GEO

Magnaporthe oryzae Gene Expression During Biotrophic Invasion of Rice (Agilent Array, G4137B)

2009-04-08 | GSE8517 | GEO