Project description:This study aimed to investigate transcriptional differences between liver tumours that were chemically-induced in five strains and species of mouse and rat. Male mice (Mus musculus domesticus C3H/HeOuJ and C57BL/6J, Mus musculus castaneus CAST/EiJ, and Mus Caroli CAROLI/EiJ) and rats (Fischer F344) were treated with diethylnitrosamine (DEN) to induce liver tumours. After tumour dissection, genomic DNA and RNA were simultaneously isolated and purified for library preparation and sequencing. Additional liver tissue samples were collected and processed in parallel: untreated liver from infant mice (15-day old, P15) and juvenile rats (56-day old, P56); untreated adult liver tissue; DEN-exposed adult liver tissue; spontaneous liver tumours.
Project description:We compared gene expression differences in the polytypic species complex Mus musculus (Mus musculus musculus, Mus musculus domesticus, Mus musculus castaneus and Mus musculus ssp) with that of Mus spretus via oligonucleotide microarrays representing more than 20,000 genes. Analysis of the results by two way ANOVA statistics suggests that the most genes with significant differences in expression levels among the subspecies are found in liver and kidney and the least in testis. This picture is different when one compares with Mus spretus, where the largest number of differences is found in testis. Keywords: multi-species comparison