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Proteomic profiling of jejunal tissues from ETEC-challenged yak calves supplemented with Pediococcus pentosaceus NQ0729

ABSTRACT: This study generated quantitative proteomic data to investigate protein expression changes in the jejunum of yak calves following enterotoxigenic Escherichia coli (ETEC) challenge and Pediococcus pentosaceus NQ0729 supplementation. Yak calves were divided into three groups: a control group (CON), an ETEC-challenged group (ETEC), and a P. pentosaceus NQ0729-pretreated group followed by ETEC challenge (PET). Jejunal tissue samples were collected at the end of the animal trial and analyzed using LC-MS/MS-based quantitative proteomics. The dataset was used to compare protein abundance profiles among the three groups and to identify differentially expressed proteins and enriched biological pathways associated with ETEC challenge and probiotic intervention in yak calves.

ORGANISM(S): Bos Grunniens

SUBMITTER: Jiang Hu

PROVIDER: PXD079365 | iProX | Sat Jun 06 00:00:00 BST 2026

REPOSITORIES: iProX

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Project description:Background and Aims: Although the zinc finger transcription factor GATA4 has been implicated in regulating jejunal gene expression, the contribution of GATA4 in controlling jejunal physiology has not been addressed. Methods: We generated mice in which the Gata4 gene was specifically deleted in the small intestinal epithelium. Measurements of plasma cholesterol and phospholipids, intestinal absorption of dietary fat and cholesterol, and gene expression were performed on these animals. Results: Mice lacking GATA4 in the intestine displayed a dramatic block in their ability to absorb cholesterol and dietary fat. Comparison of the global gene expression profiles of control jejunum, control ileum, and GATA4 null jejunum by gene array analysis demonstrated that GATA4 null jejunum lost expression of 53% of the jejunal-specific gene set and gained expression of 47% of the set of genes unique to the ileum. These alterations in gene expression included a decrease in mRNAs encoding lipid and cholesterol transporters as well as an increase in mRNAs encoding proteins involved in bile acid absorption. Conclusion: Our data demonstrate that GATA4 is essential for jejunal function including fat and cholesterol absorption and confirm that GATA4 plays a pivotal role in determining jejunal versus ileal identity. Experiment Overall Design: Total RNA was harvested from the following sources and used to Affymetrix array analysis following manufacturer defined protocols: Experiment Overall Design: control jejunum (Gata4loxP/+VilCre), 3 male mice, adult (6-8 wk) Experiment Overall Design: mutant jejunum (Gata4loxP/-VilCre), 3 male mice, adult (6-8 wk) Experiment Overall Design: control ileum (Gata4loxP/+VilCre), 3 male mice, adult (6-8 wk). Experiment Overall Design: A total of nine Mouse Genome 430_2.0 arrays were hybridized for this study. Experiment Overall Design: Jejunum was defined as 10 cm from the pyloric sphincter, and ileum was defined as 1 cm from the cecum. The animals used to harvest control jejunum and ileum were independent of each other.

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Proteomic profiling of jejunal tissues from ETEC-challenged yak calves supplemented with Pediococcus pentosaceus NQ0729

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