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Dissection of In Vivo Archaeal RNA Polymerase Assembly Reveals a Modular Pathway and Stoichiometric Control

ABSTRACT: These findings establish a modular and hierarchical framework for archaeal RNAP assembly, uncover key regulatory checkpoints, and demonstrate the essential role of small subunit stoichiometry. Given the shared subcomplex architecture and assembly logic, these insights not only fill a longstanding knowledge gap in archaeal RNAP assembly but also illuminate its evolutionary continuity with bacterial and eukaryotic systems and uncover regulatory vulnerabilities with potential applied relevance.

ORGANISM(S): Methanococcus Maripaludis X1

SUBMITTER: Jie Li

PROVIDER: PXD081683 | iProX | Mon Jul 27 00:00:00 BST 2026

REPOSITORIES: iProX

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Dissection of in vivo archaeal RNA polymerase assembly reveals a modular pathway and stoichiometric control.

Li Jie J Qi Wen W Duan Huaping H Ren Derong D Liu Jingfang J Dong Xiuzhu X

mBio 20260728

Archaeal transcription is driven by a multi-subunit RNA polymerase (RNAP) structurally and functionally homologous to eukaryotic RNAP II. However, its in vivo biogenesis remains uncharacterized. Here, we present the first comprehensive dissection of archaeal RNAP assembly using Methanococcus maripaludis as a genetically tractable model. Through an integrative strategy combining size exclusion chromatography, native purification, Western blotting, mass spectrum, and in vitro ...[more]

PMID: 42517641

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