Proteomics

Dataset Information

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Human iPS cell proteome

ABSTRACT: Rapid and deep profiling of human induced pluripotent stem cell proteome analysis by one-shot nano LC-MS/MS with meter-scale monolithic silica capillary columns.

ORGANISM(S): Homo Sapiens (human)

SUBMITTER: Yasushi Ishihama 

PROVIDER: PXD004528 | JPOST Repository | Mon Jun 20 00:00:00 BST 2016

REPOSITORIES: jPOST

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Publications

Rapid and deep profiling of human induced pluripotent stem cell proteome by one-shot NanoLC-MS/MS analysis with meter-scale monolithic silica columns.

Yamana Ryota R   Iwasaki Mio M   Wakabayashi Masaki M   Nakagawa Masato M   Yamanaka Shinya S   Ishihama Yasushi Y  

Journal of proteome research 20121204 1

Proteome analyses of human induced pluripotent stem cells (iPSC) were carried out on a liquid chromatography-tandem mass spectrometry system using meter-scale monolithic silica-C18 capillary columns without prefractionation. Tryptic peptides from five different iPSC lysates and three different fibroblast lysates (4 μg each) were directly injected onto a 200 cm long, 100 μm i.d. monolithic silica-C18 column and an 8-h gradient was applied at 500 nL/min at less than 20 MPa. We identified 98,977 no  ...[more]