Proteomics

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TIG-3 quantification of metabolic enzymes set05

ABSTRACT: Absolute quantification of metabolic enzymes (core metabolic set) was carried out by MRM with internal standard recombinant proteins. Sample digests were labeled with mTRAQdelta0 and internal standard digests were labeled with mTRAQdelta4.

ORGANISM(S): Homo Sapiens (human)

SUBMITTER: Masaki Matsumoto 

PROVIDER: PXD004833 | JPOST Repository | Sat Dec 03 00:00:00 GMT 2016

REPOSITORIES: jPOST

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Targeted proteomics approaches are of value for deep and accurate quantification of protein abundance. Extending such methods to quantify large numbers of proteins requires the construction of predefined targeted assays. We developed a targeted proteomics platform-in vitro proteome-assisted multiple reaction monitoring (MRM) for protein absolute quantification (iMPAQT)-by using >18,000 human recombinant proteins, thus enabling protein absolute quantification on a genome-wide scale. Our platform  ...[more]

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