Sequential Phosphoproteomic Enrichment through Complementary Metal-Directed Immobilized Metal Ion Affinity Chromatography

ABSTRACT: We developed a tip-based sequential IMAC based on different kinds of metal ion for increasing the coverage of phosphoproteome. This approach not only can be used for singly and multiply phosphopeptides separation but also for acidic, basophilic and Pro-directed phosphopeptides separation.

ORGANISM(S): Homo Sapiens (human)

SUBMITTER: Chia-Feng Tsai, Chuan-Chih Hsu and Yu-Ju Chen 

PROVIDER: PXD004903 | JPOST Repository | Wed Sep 07 00:00:00 BST 2016

REPOSITORIES: jPOST