Rapid High-pH Reverse Phase StageTip for Sensitive Small-Scale Membrane Proteomic Profiling

ABSTRACT: To increase the coverage of membrane protein identification,we developed high-pH reverse phase (Hp-RP) combined with stop-and-go extraction tip (StageTip) technique, as a fast (∼15 min.). Almost 5000 proteins (∼60% membrane proteins) can be identified in only 10 μg of membrane protein digests, showing the superior sensitivity of the Hp-RP StageTip approach.

ORGANISM(S): Homo Sapiens (human)

SUBMITTER: Baby Rorielyn T. Dimayacyac-Esleta and Yu-Ju Chen 

PROVIDER: PXD004904 | JPOST Repository | Thu Sep 08 00:00:00 GMT+01:00 2016

REPOSITORIES: jPOST