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Ampulla of Poecilimon ampliatus liquid sample

ABSTRACT: The spermatophore was removed and separated into ampulla and spermatophylax. Each of these compartments was solubilized in 6M urea, 3 M thiourea, 70 mM DTT and centrifuged. The supernatant was analyzed directly by LC-FTICR MS.

ORGANISM(S): Poecilimon Ampliatus

SUBMITTER: Peter Jungblut

PROVIDER: PXD005029 | JPOST Repository | Sat Sep 23 00:00:00 BST 2017

REPOSITORIES: jPOST

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Dataset's files

Source:

			Action	DRS
	140614_Lehmann_Ampulle.mzXML	Mzxml
	Ampulle_liquid.xml	Xml
	LTQ_140614_Lehmann_Ampulle_liquid.mgf	Mgf

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Project description:The spermatophore was removed and separated into ampulla and spermatophylax. Each of these compartments was solubilized in 6M urea, 3 M thiourea, 70 mM DTT and centrifuged. The supernatant was separated by 2-DE and spots 9,10, and 11 were analyzed by LC-FTICR MS. Spots 9,10, and 11 were occuring on 2-DE patterns of both spermatophylax and ampulla.

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