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Optimization of Phosphotyrosine Proteomics

ABSTRACT: We optimized a procedure of phosphotyrosine enrichment with immunoprecipitation. Then the optimized procedure was applied into a global analysis of phosphotyrosine status in culture cells treated with Erlotinib.

ORGANISM(S): Homo Sapiens (human)

SUBMITTER: Takeshi Tomonaga

PROVIDER: PXD005061 | JPOST Repository | Fri Sep 29 00:00:00 BST 2017

REPOSITORIES: jPOST

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			Action	DRS
	HCT2mg_fig1_A_01.raw	Raw
	HCT2mg_fig1_A_02.raw	Raw
	HCT2mg_fig1_A_03.raw	Raw
	HCT2mg_fig1_B_01.raw	Raw
	HCT2mg_fig1_B_02.raw	Raw

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Publications

Deep Phosphotyrosine Proteomics by Optimization of Phosphotyrosine Enrichment and MS/MS Parameters.

Abe Yuichi Y Nagano Maiko M Tada Asa A Adachi Jun J Tomonaga Takeshi T

Journal of proteome research 20161205 2

Phosphorylation is a major post-translational modification that regulates protein function, with phosphotyrosine (pY) modifications being implicated in oncogenesis. However, global profiling of pY statuses without treatment with a tyrosine phosphatase inhibitor such as pervanadate is still challenging due to the low occupancy of pY sites. In this study, we greatly improved the identification of pY sites by liquid chromatography-tandem mass spectrometry (LC-MS/MS) by optimization of both the pY-i ...[more]

PMID: 28152594

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