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BONCAT enables time-resolved analysis of protein synthesis in native plant tissue

ABSTRACT: Pulsing the non-canonical amino acid azidohomoalanine into Arabidopsis thaliana seedlings enables in-gel visualization, physical enrichment, and identification of newly synthesized proteins.

ORGANISM(S): Arabidopsis Thaliana (mouse-ear Cress)

SUBMITTER: David Tirrell

PROVIDER: PXD005577 | JPOST Repository | Mon Jul 03 00:00:00 BST 2017

REPOSITORIES: jPOST

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Dataset's files

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			Action	DRS
	Wes_HS1.raw	Raw
	Wes_HS2.raw	Raw
	Wes_HS3.raw	Raw
	Wes_Recovery1.raw	Raw
	Wes_Recovery2.raw	Raw

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Publications

Bioorthogonal Noncanonical Amino Acid Tagging (BONCAT) Enables Time-Resolved Analysis of Protein Synthesis in Native Plant Tissue.

Glenn Weslee S WS Stone Shannon E SE Ho Samuel H SH Sweredoski Michael J MJ Moradian Annie A Hess Sonja S Bailey-Serres Julia J Tirrell David A DA

Plant physiology 20170119 3

Proteomic plasticity undergirds stress responses in plants, and understanding such responses requires accurate measurement of the extent to which proteins levels are adjusted to counter external stimuli. Here, we adapt bioorthogonal noncanonical amino acid tagging (BONCAT) to interrogate protein synthesis in vegetative Arabidopsis (<i>Arabidopsis thaliana</i>) seedlings. BONCAT relies on the translational incorporation of a noncanonical amino acid probe into cellular proteins. In this study, the ...[more]

PMID: 28104718

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Time-Resolved Proteomics Extends Ribosome Profiling-Based Measurements of Protein Synthesis Dynamics

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