Phosphoprotoemics analysis of FAO cells stimulated with insulin

ABSTRACT: FAO cells, rat hepatoma cells, were stimulated with 1 nM insulin and collected at seven time points for ~60 minutes. Ion metal affinity chromatography (IMAC) was performed to enrich the phosphopeptides. As we used two sets of 4-plex iTRAQ to quantify the peptides from seven samples, we set the internal control to calibrate two sets of phosphoproteomics data

ORGANISM(S): Rattus Rattus (black Rat)

SUBMITTER: Sinya Kuroda 

PROVIDER: PXD005900 | JPOST Repository | Fri Nov 30 00:00:00 GMT 2018

REPOSITORIES: jPOST