Proteomics

Dataset Information

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SWATH-MS in TCTP-deficient MPNST cells

ABSTRACT: EF1A2-binding proteins in TCTP-deficient MPNST cells were identified by immuno-precipitation using anti-FLAG antibody and SWATH. To verify whether the change of the amount of EF1A2-binding proteins caused the cellular expression change, the proteins expressed in sNF96.2 MPNST cells treated with TCTP-siRNA were profiled by SWATH-MS.

ORGANISM(S): Homo Sapiens (human)

SUBMITTER: Norie Araki 

PROVIDER: PXD007606 | JPOST Repository | Mon Nov 05 00:00:00 GMT 2018

REPOSITORIES: jPOST

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Publications

Identification of a Specific Translational Machinery via TCTP-EF1A2 Interaction Regulating NF1-associated Tumor Growth by Affinity Purification and Data-independent Mass Spectrometry Acquisition (AP-DIA).

Kobayashi Daiki D   Tokuda Takaho T   Sato Kyosuke K   Okanishi Hiroki H   Nagayama Megumi M   Hirayama-Kurogi Mio M   Ohtsuki Sumio S   Araki Norie N  

Molecular & cellular proteomics : MCP 20181031 2

Neurofibromatosis type 1 (NF1) is an autosomal dominant disease that predisposes individuals to developing benign neurofibromas and malignant peripheral nerve sheath tumors (MPNST). The mechanism of NF1-tumorigenesis or the curatives have not been established. Using unique trascriptome and proteome integration method, iPEACH (1), we previously identified translationally controlled tumor protein (TCTP) as a novel biological target for NF1-associated tumors (2). Here, we identified specific TCTP-i  ...[more]