GFP-Trap-IP-MS

ABSTRACT: To analyze proteins copurified with GFP-ALLO-1, worm eggs expressing GFP-ALLO-1 or GFP in the atg-11 background were lysed in co-IP buffer, and GFP-ALLO-1 or GFP was immunoprecipitated with GFP-Trap-agarose beads. Proteins on the beads were directly digested with Trypsin overnight. Supernatants were collected, acidified to pH2.5 and desalted using GL-Tip SDB followed by LC-MS/MS analysis.

ORGANISM(S): Caenorhabditis Elegans

SUBMITTER: Hidetaka Kosako 

PROVIDER: PXD007847 | JPOST Repository | Wed Jan 31 00:00:00 GMT 2018

REPOSITORIES: jPOST