Sensitive analysis of protein tyrosine phosphorylation
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ABSTRACT: We immobilized SH2 superbinder onto a monolithic capillary column to construct a microreactor to enrich pTyr peptides from minute amount of samples. To evaluate the feasibility of this strategy, we used protein standards, phosphopeptide samples purified from pervanadate-treated HeLa cell digests as well as a protein complex sample. The results showed that this microreactor enabled highly specific enrichment of phosphotyrosine peptides.
ORGANISM(S): Homo Sapiens (human)
SUBMITTER: Mingliang Ye
PROVIDER: PXD007998 | JPOST Repository | Sat Oct 20 00:00:00 BST 2018
REPOSITORIES: jPOST
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