Sensitive analysis of protein tyrosine phosphorylation

ABSTRACT: We immobilized SH2 superbinder onto a monolithic capillary column to construct a microreactor to enrich pTyr peptides from minute amount of samples. To evaluate the feasibility of this strategy, we used protein standards, phosphopeptide samples purified from pervanadate-treated HeLa cell digests as well as a protein complex sample. The results showed that this microreactor enabled highly specific enrichment of phosphotyrosine peptides.

ORGANISM(S): Homo Sapiens (human)

SUBMITTER: Mingliang Ye 

PROVIDER: PXD007998 | JPOST Repository | Sat Oct 20 00:00:00 BST 2018

REPOSITORIES: jPOST