Perturbation-based proteomic correlation profiling as a novel target deconvolution methodology
Ontology highlight
ABSTRACT:
ORGANISM(S): Homo Sapiens (human)
SUBMITTER: Kazuishi Kubota
PROVIDER: PXD008086 | JPOST Repository | Wed Jul 10 00:00:00 BST 2019
REPOSITORIES: jPOST
Publications
Perturbation-Based Proteomic Correlation Profiling as a Target Deconvolution Methodology.
Ohki Yu Y Sakurai Hidetaka H Hoshino Madoka M Terashima Hideki H Shimizu Hiroki H Ishikawa Tomio T Ogiyama Tomoko T Muramatsu Yasunori Y Nakanishi Toshiyuki T Miyazaki Shojiro S Tsuruoka Hiroyuki H Kobayashi Hideki H Kubota Kazuishi K
Cell chemical biology 20181115 1
Molecular target identification of small molecules, so-called target deconvolution, is a major obstacle to phenotype-based drug discovery. Here, we developed an approach called perturbation-based proteomic correlation profiling (PPCP) utilizing the correlation between protein quantity and binding activity of compounds under cellular perturbation by gene silencing and successfully identified lanosterol synthase as a molecular target of TGF-β pathway inhibitor. This PPCP concept was extended to th ...[more]
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