Dataset Information

K562 Elutriation cell cycle phase fractions 2D-MS-CETSA

ABSTRACT: K562 cells were synchronized by cell size difference based Elutriation scheme into G1, S and G2 fractions. Three biological replicates were collected on different days, and assembled into a 2D-CETSA scheme with 6 different heating temperatures (37ºC, 47ºC, 50ºC, 52ºC,54ºC and 57ºC), (Figure 1A, Methods section of the associated paper), with each set of samples heated at one temperature included in the same isobaric TMT10 set and measured at the same time on Mass Spectrometer. The labeling order of samples in the TMT10 channels is as follows: 37C/47C samples: Biorep1_G1(126), Biorep1_S(127N), Biorep1_G2(127C), Biorep2_G1(128N), Biorep2_S(128C), Biorep2_G2(129N), Biorep3_S(129C), Biorep3_G1(130N), Biorep3_G2(130C), mixed_control(131); 50C/52C/54C/57C samples: Biorep1_G1(126), Biorep1_S(127N), Biorep1_G2(127C), Biorep2_G1(128N), Biorep2_S(128C), Biorep2_G2(129N), Biorep3_G1(129C), Biorep3_S(130N), Biorep3_G2(130C), mixed_control(131);

ORGANISM(S): Homo Sapiens (human)

SUBMITTER: Pär Nordlund

PROVIDER: PXD009171 | JPOST Repository | Tue May 08 00:00:00 BST 2018

REPOSITORIES: jPOST

ACCESS DATA

Dataset's files

Source:

			Action	DRS
	20170414_LY_TY_Elutri_54C_3bio_rep1_1.raw	Raw
	20170414_LY_TY_Elutri_54C_3bio_rep1_10.raw	Raw
	20170414_LY_TY_Elutri_54C_3bio_rep1_11.raw	Raw
	20170414_LY_TY_Elutri_54C_3bio_rep1_12.raw	Raw
	20170414_LY_TY_Elutri_54C_3bio_rep1_13.raw	Raw

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Publications

Modulation of Protein-Interaction States through the Cell Cycle.

Dai Lingyun L Zhao Tianyun T Bisteau Xavier X Sun Wendi W Prabhu Nayana N Lim Yan Ting YT Sobota Radoslaw M RM Kaldis Philipp P Nordlund Pär P

Cell 20180426 6

Global profiling of protein expression through the cell cycle has revealed subsets of periodically expressed proteins. However, expression levels alone only give a partial view of the biochemical processes determining cellular events. Using a proteome-wide implementation of the cellular thermal shift assay (CETSA) to study specific cell-cycle phases, we uncover changes of interaction states for more than 750 proteins during the cell cycle. Notably, many protein complexes are modulated in specifi ...[more]

PMID: 29706543

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