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Cytoplasmic capping enzyme interactome

ABSTRACT: Formaldehyde crosslinking and Proximity-dependent biotinylation methods were applied to study the cytoplasmic capping enzyme interactome via 1D-LC-MS/MS. The raw data were searched against Uniprot human database modified to contain our designed protein sequences using the Thermo Proteome Discoverer software (v 1.4.1.14).

ORGANISM(S): Homo Sapiens (human)

SUBMITTER: Daniel Schoenberg

PROVIDER: PXD010368 | JPOST Repository | Thu Aug 30 00:00:00 BST 2018

REPOSITORIES: jPOST

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RNA-binding proteins and heat-shock protein 90 are constituents of the cytoplasmic capping enzyme interactome.

Trotman Jackson B JB Agana Bernice A BA Giltmier Andrew J AJ Wysocki Vicki H VH Schoenberg Daniel R DR

The Journal of biological chemistry 20180830 43

The <i>N</i><sup>7</sup>-methylguanosine cap is added in the nucleus early in gene transcription and is a defining feature of eukaryotic mRNAs. Mammalian cells also possess cytoplasmic machinery for restoring the cap at uncapped or partially degraded RNA 5' ends. Central to both pathways is capping enzyme (CE) (RNA guanylyltransferase and 5'-phosphatase (RNGTT)), a bifunctional, nuclear and cytoplasmic enzyme. CE is recruited to the cytoplasmic capping complex by binding of a C-terminal proline- ...[more]

PMID: 30166341

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