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Hematopoietic stem cells (HSCs)

ABSTRACT: G0 marker-separation of dormant and active hematopoietic stem cells reveals dormancy regulation by basal cytoplasmic calcium

OTHER RELATED OMICS DATASETS IN: GSE139013GSE138884

ORGANISM(S): Mus Musculus (mouse)

SUBMITTER: Toshio Kitamura

PROVIDER: PXD012093 | JPOST Repository | Thu Dec 19 00:00:00 GMT 2019

REPOSITORIES: jPOST

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Discrimination of Dormant and Active Hematopoietic Stem Cells by G0 Marker Reveals Dormancy Regulation by Cytoplasmic Calcium.

Fukushima Tsuyoshi T Tanaka Yosuke Y Hamey Fiona K FK Chang Chih-Hsiang CH Oki Toshihiko T Asada Shuhei S Hayashi Yasutaka Y Fujino Takeshi T Yonezawa Taishi T Takeda Reina R Kawabata Kimihito Cojin KC Fukuyama Tomofusa T Umemoto Terumasa T Takubo Keiyo K Takizawa Hitoshi H Goyama Susumu S Ishihama Yasushi Y Honda Hiroaki H Göttgens Berthold B Kitamura Toshio T

Cell reports 20191201 12

Quiescent hematopoietic stem cells (HSCs) are typically dormant, and only a few quiescent HSCs are active. The relationship between "dormant" and "active" HSCs remains unresolved. Here we generate a G0 marker (G0M) mouse line that visualizes quiescent cells and identify a small population of active HSCs (G0Mlow), which are distinct from dormant HSCs (G0Mhigh), within the conventional quiescent HSC fraction. Single-cell RNA-seq ana ...[more]

PMID: 31851939

Similar Datasets

Discrimination of dormant and active hematopoietic stem cells by G0 markers reveals dormancy regulation by cytoplasmic calcium

Project description:Discrimination of dormant and active hematopoietic stem cells by G0 markers reveals dormancy regulation by cytoplasmic calcium

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Discrimination of dormant and active hematopoietic stem cells by G0 markers reveals dormancy regulation by cytoplasmic calcium

Project description:Discrimination of dormant and active hematopoietic stem cells by G0 markers reveals dormancy regulation by cytoplasmic calcium

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Discrimination of dormant and active hematopoietic stem cells by G0 markers reveals dormancy regulation by cytoplasmic calcium

Project description:Quiescent hematopoietic stem cells (HSCs) are typically dormant, and only a few quiescent HSCs are active. The relationship between “dormant” and “active” HSCs remains unresolved. Here we generated a G0 marker (G0M) mouse line that visualizes quiescent cells. G0M identifies a small population of active HSCs (G0Mlow), which are distinct from dormant HSCs (G0Mhigh), within the conventional quiescent HSC fraction. Single-cell RNA-Seq analyses showed that the gene expression profiles of these populations were nearly identical, yet differed in their Cdk4/6 activity. Furthermore high-throughput small molecule screening revealed that high concentrations of cytoplasmic calcium ([Ca2+]c) were linked to dormancy of HSCs. These findings indicate that G0M separates dormant and active adult HSCs, which are regulated by Cdk4/6 and [Ca2+]c. This G0M mouse line represents a useful resource for investigating physiologically important stem cell subpopulations.

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