TMT-based quantitative MS analysis of proteome labeled by NO-responsive reaction with NOAR-2

ABSTRACT: RAW264.7 cells were treated by LPS and IFN-gamma for 20 h. The LPS/IFN treated or nontreated cells were labeled by NOAR-2 for 1 h. Cells were lysed by NP-40 buffer. Labeled proteins were purified by immunopecipitation using anti-fluorescein, then digested by trypsin. Digested peptides were labeled by TMTsixplex isobaric labeling reagents, followed by mass spectrometry measurement.

ORGANISM(S): Mus Musculus (mouse)

SUBMITTER: Itaru Hamachi 

PROVIDER: PXD012310 | JPOST Repository | Sun Jan 12 00:00:00 GMT 2020

REPOSITORIES: jPOST