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Age-associated mouse brown adipose tissue mitochondrial proteome

ABSTRACT: We investigated the age-associated thermogenesis activation in brown fat mitochondria. We administered the young mice (10 weeks old) and old mice (76 weeks old) with a selective β3-adrenergic receptor agonist (β3-AR agonist, CL316,243) at a high dose (1 mg kg-1) for five days at 30˚C to activate thermogenesis. We isolated mitochondria from brown fat for proteome profiling. We applied 250 ng peptides for LC/MS/MS analysis using label-free quantification coupled match-between-run approach.

ORGANISM(S): Mus Musculus (mouse)

SUBMITTER: Shingo Kajimura

PROVIDER: PXD013410 | JPOST Repository | Thu Jul 18 00:00:00 BST 2019

REPOSITORIES: jPOST

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