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Effect of the ribonuclease binase on the proteome of A549 cell infected by influenza A(H1N1pdm09) virus

ABSTRACT: Ribonucleases (RNases) of different origin demonstrate antiviral effects that are mediated by the direct RNase action on RNA species and can also involve signal transduction modulation. To reveal cellular targets and explain the mechanisms underlying the antiviral effect employed by the small extra-cellular ribonuclease of Bacillus pumilus (binase) both in vitro and in vivo, a shotgun proteomic analysis of influenza A virus (H1N1pdm09)-infected A549 cells upon binase treatment, was performed.

ORGANISM(S): Homo Sapiens (human)

SUBMITTER: Raihan Shah Mahmud

PROVIDER: PXD013545 | JPOST Repository | Thu Apr 18 00:00:00 BST 2019

REPOSITORIES: jPOST

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			Action	DRS
	Raich_1_Kontr_RA3_01_1760.baf	Other
	Raich_1_Kontr_RA3_01_1760.mgf	Mgf
	Raich_1_Kontr_RA3_01_1760.mzid	Mzid
	Raich_2_kl_RNAse_RA4_01_1762.baf	Other
	Raich_2_kl_RNAse_RA4_01_1762.mgf	Mgf

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Anti-Influenza Activity of the Ribonuclease Binase in A549 cells

Project description:Ribonucleases (RNases) of different origin demonstrate antiviral effects that are mediated by the direct RNase action on RNA species and can also involve signal transduction modulation. To reveal cellular targets and explain the mechanisms underlying the antiviral effect employed by the small extra-cellular ribonuclease of Bacillus pumilus (binase) both in vitro and in vivo, a shotgun proteomic analysis of influenza A virus (H1N1pdm09)-infected A549 cells upon binase treatment, was performed.

2019-04-17 | PXD013540 | JPOST Repository

Effect of the ribonuclease binase on the proteome of A549 cell infected by influenza A(H1N1pdm09) virus

Project description:Ribonucleases (RNases) of different origin demonstrate antiviral effects that are mediated by the direct RNase action on RNA species and can also involve signal transduction modulation. To reveal cellular targets and explain the mechanisms underlying the antiviral effect employed by the small extracellular ribonuclease of Bacillus pumilus (binase) both in vitro and in vivo, a shotgun proteomic analysis of influenza A virus (H1N1pdm09)-infected A549 cells upon binase treatment, was performed.

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Initiation of a ZAKa-dependent Ribotoxic Stress Response by the Innate Immunity Endoribonuclease RNase L

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RNase L regulates the antiviral proteome by accelerating mRNA decay, inhibiting nuclear mRNA export, and repressing RNAPII-mediated transcription

Project description:Ribonuclease L (RNase L) is an antiviral endoribonuclease that triggers widespread degradation of cellular mRNAs. Here, we show that the degradation of cellular mRNA by RNase L is a conserved response to flaviviruses, including Zika virus (ZIKV), dengue virus serotype 2 (DENV-2), and West Nile virus (WNV). Quantitative mass spectrometry in response to dsRNA or ZIKV infection shows that RNase L broadly downregulates the cellular proteome, reducing proteins with short half-lives involved in cell cycle progression, cellular metabolism, and protein synthesis. The mRNAs encoded by interferon-stimulated genes (ISGs) evade mRNA decay by RNase L, allowing for protein synthesis of ISG-encoding mRNAs. However, RNase L dampens ISG protein synthesis by triggering a block in nuclear mRNA export and repressing RNAPII-mediated transcription at later times during the antiviral response. These findings implicate reprograming of the cellular proteome as primary means by which RNase L combats viral infection, tumorigenesis, and immune dysregulation.

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