Proteomics

Dataset Information

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HUVEC mCherry or GPNMB-mChery over expressed, mCherry-IP


ABSTRACT: HUVEC were infected retroviral vectors expressing mCherry-GPNMB or mCherry. The cells were lysed with Pierce IP lysis buffer. The lysates after immunoprecipitation using anti-mCherry antibody (ab213511, Abcam) was transferred into a new microcentrifuge tube for “tube gel digestion”, a modified in-gel digestion procedure, to generate dithiothreitol-reduced iodoacetamide-alkylated tryptic peptides for nano-flow liquid chromatography coupled with tandem mass spectrometry (LC-MS/MS).

ORGANISM(S): Homo Sapiens (human)

SUBMITTER: Tohru Minamino 

PROVIDER: PXD013762 | JPOST Repository | Sun May 08 00:00:00 BST 2022

REPOSITORIES: jPOST

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