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Proteomics of human TB and MAC-LD granulomas

ABSTRACT: FFPE of TB or MAC-LD granulomatous lesions from surgically resection were dissected and subcompartments of granulomas were separately collected. Tryptic peptides were subjected to a Q Exactive and proteomic data were acquired.

ORGANISM(S): Homo Sapiens (human)

SUBMITTER: Shintaro Seto

PROVIDER: PXD014086 | JPOST Repository | Fri Nov 19 00:00:00 GMT 2021

REPOSITORIES: jPOST

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Proteomic Profiling Reveals the Architecture of Granulomatous Lesions Caused by Tuberculosis and Mycobacterium avium Complex Lung Disease.

Seto Shintaro S Morimoto Kozo K Yoshida Tsutomu T Hiramatsu Miyako M Hijikata Minako M Nagata Toshi T Kikuchi Fumihito F Shiraishi Yuji Y Kurashima Atsuyuki A Keicho Naoto N

Frontiers in microbiology 20200117

Tuberculosis (TB) and Mycobacterium avium complex lung disease (MAC-LD) are both characterized pathologically by granuloma lesions, which are typically composed of a necrotic caseum at the center surrounded by fibrotic cells and lymphocytes. Although the histological characterization of TB and MAC-LD granulomas has been well-documented, their molecular signatures have not been fully evaluated. In this research we applied mass spectrometry-based proteomics combined with laser microdissecti ...[more]

PMID: 32010116

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