Proteomics

Dataset Information

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BioID2_BirA-MAVS_CLPB-BirA_PHB1-BirA_HEK293

ABSTRACT: HEK293 cells that stably express MAVS, CLPB or PHB1 fused at C-terminus with the biotin ligase from A. aeolicus were incubated with 100 μM biotin for 18 h. The mitochondrial fractions were lysed in RIPA buffer, and the clarified supernatants were incubated with streptavidin agarose beads for 2 h at 4° C. Proteins on the beads were digested by adding trypsin/Lys-C mix for 16 h at 37°C and analyzed by LC-MS/MS. Label-free quantification was performed using Proteome Discoverer 2.2 software.

ORGANISM(S): Homo Sapiens (human)

SUBMITTER: Hidetaka Kosako 

PROVIDER: PXD014218 | JPOST Repository | Thu Sep 05 00:00:00 BST 2019

REPOSITORIES: jPOST

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Dataset's files

Source:
Action DRS
BioID2_CLPB.raw Raw
BioID2_Control.raw Raw
BioID2_MAVS.raw Raw
BioID2_PHB1.raw Raw
MAVS_CLPB_PHB1_BioID2.xlsx Xlsx
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Publications

Vessel dilator, long acting natriuretic peptide, and kaliuretic peptide increase circulating prostaglandin E2.

Vesely D L DL   Perez-Lamboy G I GI   Schocken D D DD  

Life sciences 20000101 10

Prostaglandin E2 (PGE2) increases in the circulation of persons with congestive heart failure (CHF), but the cause of this increase is unknown. Prostaglandins are not stored, therefore, they cannot be released in response to congestive heart failure itself but rather need to have their synthesis stimulated by a hormone or some other substance. Prostaglandin E2's biologic properties are nearly identical to four peptide hormones originating from amino acids 1-30 [long acting natriuretic peptide],  ...[more]