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AN01_Rattus

ABSTRACT: Identification by nano-LC-MS/MS of NT5DC2 as a protein binding to tyrosine hydroxylase: Down-regulation of NT5DC2 by siRNA increases catecholamine synthesis in PC12D cells

ORGANISM(S): Rattus Norvegicus

SUBMITTER: Akira Nakashima

PROVIDER: PXD014417 | JPOST Repository | Sat Jul 20 00:00:00 BST 2019

REPOSITORIES: jPOST

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Identification by nano-LC-MS/MS of NT5DC2 as a protein binding to tyrosine hydroxylase: Down-regulation of NT5DC2 by siRNA increases catecholamine synthesis in PC12D cells.

Nakashima Akira A Yamaguchi Hisateru H Kodani Yu Y Kaneko Yoko S YS Kawata Miho M Nagasaki Hiroshi H Nagatsu Toshiharu T Ota Akira A

Biochemical and biophysical research communications 20190703 4

Tyrosine hydroxylase (TH), which catalyzes the conversion of l-tyrosine to l-DOPA, is the rate-limiting enzyme in the biosynthesis of catecholamines. It is well known that both α-synuclein and 14-3-3 protein family members bind to the TH molecule and regulate phosphorylation of its N-terminus by kinases to control the catalytic activity. In this present study we investigated whether other proteins aside from these 2 proteins might also bind to TH molecules. Nano-LC-MS/MS analysis revealed that 5 ...[more]

PMID: 31279527

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