Proteomics

Dataset Information

Optimization of data-independent acquisition-MS for deep proteome analysis

ABSTRACT: Data-independent acquisition (DIA)-MS analysis overtops the existing data-dependent acquisition (DDA)-MS analysis enabling deep proteome coverage and precise relative quantitative analysis in single-shot LC-MS/MS. We constructed a single-shot LC-MS/MS system with an MS measurement time of 90 min for a highly sensitive and deep proteomic analysis by optimizing the parameters of DIA and nanoLC. Furthermore, we applied this system to the proteomic analysis of cerebrums in germ-free and specific pathogen-free mice.

ORGANISM(S): Homo Sapiens (human) Cellular Organisms Mus Musculus (mouse)

SUBMITTER: Yusuke Kawashima 

PROVIDER: PXD016032 | JPOST Repository | Mon Oct 28 00:00:00 GMT 2019

REPOSITORIES: jPOST

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Action DRS
90min_DDA_HEK293_200ng_1.raw Raw
90min_DDA_HEK293_200ng_2.raw Raw
90min_DDA_HEK293_200ng_3.raw Raw
90min_GPF1_OverlappingWindowDIA_Cerebrum_Pool_For_library.raw Raw
90min_GPF2_OverlappingWindowDIA_Cerebrum_Pool_For_library.raw Raw
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