Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Proteomic analysis in culture medium of hiPS-gut monoculture, PXB-cells monoculture, and their co-culture

ABSTRACT: Proteomic analysis revealed that expression of 120, 98, and 138 kinds of protein were increased by more than two-fold in culture medium of hiPS-gut monoculture, PXB-cells monoculture, and co-culture condition compared with non-treatment medium, respectively.

ORGANISM(S): Homo Sapiens (human) Mus Musculus (mouse)

SUBMITTER: Marie Shinohara

PROVIDER: PXD017440 | JPOST Repository | Wed Feb 10 00:00:00 GMT 2021

REPOSITORIES: jPOST

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	Co-cultuer_20190118.raw	Raw
	Co-cultuer_20190118.xlsx	Xlsx
	Control_20190118.raw	Raw
	PXB_20190118.raw	Raw
	iPS_20190118.raw	Raw

Items per page:

1 - 5 of 5

Similar Datasets

Bacteria in the culture medium

Project description:Bacteria in the culture medium Raw sequence reads

| PRJNA1184462 | ENA

In-vitro culture of single gut bacterial strains with added sugars

Project description:Gut commensal bacterial strains were cultured with or without added sugars (glucose, sucrose and kestose). The base culture medium without sugar added were modified based on the Yeast Casitone Fatty Acids (YCFA) broth. Samples were analyzed for proteomics using LC-MS/MS.

2023-06-08 | PXD037923 | Pride

In-vitro human gut microbiota culture with added sugars

Project description:Three human gut microbiome samples from different individuals were cultured in an optimized culture medium with or without the presence of different sugars (10 mM glucose, 20 mM fructose, 10 mM glucose + 20 mM fructose, or 10 mM kestose). Samples were cultured in technical triplicates, and were taken at 0 hr, 1hr, 5 hr, 12 hr and 24 hr of culturing for optical density and metaproteomic analyses. Cultured microbiota cells were subjected to metaproteomics analysis using LC-MS/MS and a TMT approach.

2024-06-16 | PXD037925 | Pride

Human embryonic stem cells in E8 and AKIT culture medium

Project description:Analysis of undifferentiated KhES-1 human embryonic stem cells in growth factors-dependent (E8) and -independent (AKIT) culture medium. Results provide insight into genes differentially expressed in pluripotent states maintained by AKIT and E8 culture medium.

2017-07-01 | GSE92628 | GEO

Evaluating in vitro culture medium of mouse gut microbiome

Project description:The mice stool samples were collected for culturing with different medium for understanding the role of different medium component on the microbiome.

2018-06-15 | PXD006808 | Pride

The effect of culture medium from apoptotic chondrocytes on macrophages

Project description:To investigate the altered gene expression levels in macrophages treated with culture medium from apoptotic chondrocytes and normal chondrocytes. Each group had a biological repeat (n=3). Results provide insight into the effect of culture medium from apoptotic chondrocytes on macrophages.

2025-04-27 | GSE201711 | GEO

Human embryonic stem cells in AKIT, E8 and KSR/bFGF culture medium

Project description:Analysis of KhES-1 and H9 human ES cells in growth factors-dependent (E8) and -independent (AKIT) medium in feeder-free culture condition and KSR/bFGF medium on a feeder-layer. Results provide insight into genetic stability in different culture media/conditions.

2017-09-08 | GSE103636 | GEO

Corynebacterium diphtheriae proteome adaptation to cell culture medium and serum

Project description:ost-pathogen interactions are often studied in vitro using primary or immortal cell lines. This set-up avoids ethical problems of animal testing and has the additional advantage of lower costs. However, the influence of cell culture media on bacterial growth and metabolism is not considered or investigated in most cases. To address this question growth and proteome adaptation of Corynebacterium diphtheriae strain ISS3319 were investigated in this study. Bacteria were cultured in standard growth medium, cell culture medium and fetal calf serum. Mass spectrometric analyses and label-free protein quantification hint to an increased bacterial pathogenicity when grown in cell culture medium as well as an influence of the growth medium on the cell envelope.

2021-03-12 | PXD024381 | Pride

Transcription profiling of A. glutinosa roots growing with Frankia culture supernatant in nitrogen-free culture medium against A. glutinosa roots alone in nitrogen-free culture medium plus frankialess culture medium for 2 days

Project description:Experiment with 2 conditions: - BAP-PCM : A. glutinosa alone in nitrogen-free culture medium plus frankialess culture medium during 2 days (reference condition). - CN BAP-PCM : A. glutinosa in nitrogen-free culture medium with Frankia culture supernatant during 2 days.

2021-12-31 | E-MTAB-8937 | biostudies-arrayexpress

Proteomic analysis of unconcentrated exovesicle-depleted cell culture medium of Norway spruce

Project description:Exovesicle-enriched fraction was isolated from the culture medium of tissue-cultured cells of Norway spruce during extracellular lignin formation by an ultracentrifugation-based protocol. From the remaining, now exovesicle-depleted culture medium, 11.8 ul of the medium was prepared for proteomic analysis.

2023-12-18 | MSV000093681 | MassIVE