Proteomics

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Identification of non-specific phosphatases in TCA cycle regulation

ABSTRACT: Protein phosphorylation is a well-established post-translational mechanism that regulates protein functions and metabolic pathways. It has been shown that several plant mitochondrial proteins are phosphorylated in a reversible manner. However, the identity of the phosphatases/kinases involved in this mechanism and their role in the regulation of the TCA cycle remains unclear. Here, we isolated and characterized plants lacking two mitochondrially targeted phosphatases (Sal2 and PP2c63) alongside pyruvate dehydrogenase kinase (PDK). Protein-protein interaction, quantitative phosphoproteomics and enzymatic analyses revealed that PDK specifically regulates pyruvate dehydrogenase (PDH), whilst Sal2 and PP2c63 regulate PDHand fumarase. Alongside with the recombinant protein complemented the purified mitochondria, the PP2c63 directly regulate the PDH and fumarase while the Sal2 indirectly regulated. Characterization of steady-state metabolite levels and fluxes in the corresponding mutants revealed that these phosphatases regulate the fluxes through the TCA cycle, with altered metabolism compromising growth of the sal2/pp2c63 double. The combined data are collectively discussed in the context of current models of the control of respiration in plants.

ORGANISM(S): Arabidopsis Thaliana (mouse-ear Cress)

SUBMITTER: Iris Finkemeier 

PROVIDER: PXD018412 | JPOST Repository | Thu Apr 01 00:00:00 BST 2021

REPOSITORIES: jPOST

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Publications

Acetylation of conserved lysines fine-tunes mitochondrial malate dehydrogenase activity in land plants.

Balparda Manuel M   Elsässer Marlene M   Badia Mariana B MB   Giese Jonas J   Bovdilova Anastasiia A   Hüdig Meike M   Reinmuth Lisa L   Eirich Jürgen J   Schwarzländer Markus M   Finkemeier Iris I   Schallenberg-Rüdinger Mareike M   Maurino Veronica G VG  

The Plant journal : for cell and molecular biology 20211110 1

Plants need to rapidly and flexibly adjust their metabolism to changes of their immediate environment. Since this necessity results from the sessile lifestyle of land plants, key mechanisms for orchestrating central metabolic acclimation are likely to have evolved early. Here, we explore the role of lysine acetylation as a post-translational modification to directly modulate metabolic function. We generated a lysine acetylome of the moss Physcomitrium patens and identified 638 lysine acetylation  ...[more]