Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

MS analysis of RNF43 phosphorylation with chymotrypsin

ABSTRACT: HA-tagged RNF43 was expressed in HEK293 cells and subjected to IP. IP product was separated by SDS-PAGE for MS analysis using chymotrypsin.

ORGANISM(S): Homo Sapiens (human)

SUBMITTER: Tadasuke Tsukiyama

PROVIDER: PXD020599 | JPOST Repository | Tue Jul 28 00:00:00 BST 2020

REPOSITORIES: jPOST

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	F072112.xml	Xml
	F072113.xml	Xml
	F072114.xml	Xml
	F072115.xml	Xml
	orb_151217_AA-TT-2_%231.raw	Raw

Items per page:

1 - 5 of 6

Similar Datasets

MS analysis of RNF43 phosphorylation

Project description:HA-tagged RNF43 was expressed in HEK293 cells and subjected to IP. IP product was separated by SDS-PAGE for MS analysis.

| PXD020598 | JPOST Repository

Identification of human PRRC2B isoform interactors

Project description:Two isoforms (Long isoform ~220 KDa, short isoform ~150 KDa) of the human PRRC2B protein (Uniprot accession Q5JSZ5) were recombinantly expressed with an HA-tag in HEK293 cell lines. The goal of the experiment is to identify differential interactors of the short vs long isoforms, in addition to their common interactors. To this aim, 3 sample types were analyzed by IP-MS: HA-tagged long PRRC2B, HA-tagged short PRRC2B, and HA-tagged mCherry (control). Four biological replicates were analyzed from each experimental group.

2025-10-26 | PXD057527 | Pride

The interactome of G3BP1 in UMUC3 cells

Project description:The HA-tagged G3BP1 complex was obtained by co-IP with anti-HA antibodies from UMUC3 cells stably expressing HA-tagged G3BP1. The purified immunoprecipitants were resolved on gradient SDS-PAGE, silver-stained, and subjected to MS analysis.

2024-05-23 | PXD035722 | Pride

Adaptor candidates

Project description:IP of candidate adaptor Ada5/Gdi1, tagged with HA, versus Mock (untagged) replicates. IP associated RNA was assayed. A replicate experimental design type is where a series of replicates are performed to evaluate reproducibility or as a pilot study to determine the appropriate number of replicates for a subsequent experiments. GeneticModification: HA-tagged candidate gene or control

2012-11-15 | GSE33747 | GEO

Gene expression changes induced by HA-PRCC-TFE3 in HEK293 cells

Project description:PRCC-TFE3 is an oncogenic chimeric transcription factor identified in human TFE3-rearranged renal cell carcinoma. To analyze gene expression changes driven by PRCC-TFE3, we generated and utilized an HA-tagged PRCC-TFE3-inducible HEK293 cell line, which expresses HA-tagged PRCC-TFE3 in a doxycycline-dependent manner. Cells were cultured with or without doxycycline, and comprehensive gene expression analysis was conducted using Affymetrix arrays .

2025-01-07 | GSE281146 | GEO

Adaptor candidates

2012-11-15 | E-GEOD-33747 | biostudies-arrayexpress

GNPS - PAP-gamma associates with PAXT nuclear exosome to control the stability of PROMPT ncRNAs

Project description:Endogenous ZFC3H1 was tagged in 293T cells. Dignam cellular extracts where subjected to sequential FLAG and HA immunoprecipitation and elution. Complexes were then analyzed using mass spectrometry at the taplin mass spectrometry facility. The 2 samples are FLA-HA IP in wild-type 293T cells and Flag-HA IP in FH-ZFC3H1 293T cells.

2022-05-16 | MSV000089482 | GNPS

Transcriptome-wide identification of RNA-binding protein and microRNA target sites by PAR-CLIP: sequencing data

Project description:We applied 4-thiouridine to cultured cells expressing the FLAG/HA-tagged RNA-binding proteins (RBPs) followed by UV 365 nm irradiation. The crosslinked RNA-protein complexes were isolated by immunoprecipitation, and the covalently bound RNA was partially digested with RNase T1 and radiolabeled. The radiolabeled RNPs were subsequenctly separated by SDS-PAGE, the crosslinked RNA segments recovered and converted into a cDNA library and sequenced.

2010-05-21 | GSE21918 | GEO

RNA-seq profiling of the Tg(myo6b:GFP-2A-rpl10a-3xHA) zebrafish hair cell IP vs. whole fish IN

Project description:We have developed and tested the efficiency of the Tg(myo6b:GFP-2A-rpl10a-3xHA) zebrafish to specifically enrich for and evaluate the translatome of inner ear and lateral line sensory hair cells (IP) compared to the whole fish transcriptome (IN). We show through RNA-seq that HA-tagged ribosome immunoprecipitation significantly enriches for RNA transcripts of known zebrafish hair cell expressed transcripts.

2018-04-24 | GSE102861 | GEO

mouse adult testis ADAD2 Immuno-precipitation and mass-spectrometry (IP-MS)

Project description:To explore the mechanism of pachytene pirna biogenesis, we employed a proteomics approach using flag-tagged adad2 transgenic mice expressed fully functional N-terminal epitope-tagged HA-ADAD2 and performed anti-FLAG immunoprecipitation coupled with quantitative mass spectrometry (IP-MS) from testis extracts of adult wt and transgenic mice.

2023-03-14 | PXD034437 | Pride

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data