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Eschericha coli proteomics with Tanaka and Tachikawa

ABSTRACT: Relative comparison of Escherichia coli proteome between wild-type and pta mutant

ORGANISM(S): Escherichia Coli

SUBMITTER: Kan Tanaka

PROVIDER: PXD020753 | JPOST Repository | Mon Jun 21 00:00:00 BST 2021

REPOSITORIES: jPOST

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Acetate overflow metabolism regulates a major metabolic shift after glucose depletion in Escherichia coli.

Shimada Tomohiro T Nakazawa Kohta K Tachikawa Tomoyuki T Saito Natsumi N Niwa Tatsuya T Taguchi Hideki H Tanaka Kan K

FEBS letters 20210630 15

Acetate overflow refers to the metabolism by which a large part of carbon incorporated as glucose into Escherichia coli cells is catabolized and excreted as acetate into the medium. We previously found that mutants for the acetate overflow pathway enzymes phosphoacetyltransferase (Pta) and acetate kinase (AckA) showed significant diauxic growth after glucose depletion in E. coli. Here, we analyzed the underlying mechanism in the pta mutant. Proteomic and other analyses revealed an increase in py ...[more]

PMID: 34125966

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