Bridgin connects the outer kinetochore to centromeric chromatin

ABSTRACT: In this project, 3XFlag-tagged protein affinity purifications were performed to identify the protein-protein interactors using Cryptococcus neoformans cell lysate. After fractionation with SDS-PAGE, peptide samples for nanoLC-MS/MS were prepared by in-gel digestion. Raw data from Orbotrap Velos pro were processed with MASCOT via Proteome discoverer2.2. Validation of peptide and protein identifications were performed with Scaffold proteome software.

ORGANISM(S): Cryptococcus Neoformans

SUBMITTER: Shreyas Sridhar 

PROVIDER: PXD021072 | JPOST Repository | Wed Sep 23 00:00:00 GMT+01:00 2020

REPOSITORIES: jPOST