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Analysis of thylakoid membrane proteome in Arabidopsis thaliana

ABSTRACT: To explore the role and target of chloroplast proteases under heat stress, thylakoid membranes were isolated from wild-type and mutant chloroplast thylakoid membrane-localized proteases after heat stress and subjected to comparative quantification by LC-MS/MS analysis using the spectral counting method.

ORGANISM(S): Arabidopsis Thaliana (mouse-ear Cress)

SUBMITTER: Kenji Nishimura

PROVIDER: PXD022899 | JPOST Repository | Sun Dec 06 00:00:00 GMT 2020

REPOSITORIES: jPOST

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Deciphering the Proteotoxic Stress Responses Triggered by the Perturbed Thylakoid Proteostasis in Arabidopsis.

Nishimura Kenji K Nakagawa Reiko R Hachisuga Chisato C Nakajima Munekage Yuri Y

Plants (Basel, Switzerland) 20210310 3

Here, we explored heat dependent thylakoid FtsH protease substrates and investigated proteotoxicity induced by thermal damage and processive protease dysfunction on the thylakoid membrane. Through our thylakoid enriched proteome analysis and biochemical experiments, carbonylated stromal proteins were suggested as possible FtsH targets. Furthermore, we observed in the thylakoid fractions in the absence of FtsH stromal reactive oxygen species-detoxifying enzymes, as well as heat shock proteins and ...[more]

PMID: 33802194

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