Peptidome analysis of the hypothalamus in a single mouse.

ABSTRACT: A 2-mg frozen section of hypothalamus from a single mouse was homogenized in 200 µL of denaturing solution(7 M urea, 2 M thiourea, 20 mM dithiothreitol) containing cOmplete Mini Protease Inhibitor Cocktail (Roche). Peptide extracts equivalent to 135 µg of tissue were analyzed by LC-MS/MS.

ORGANISM(S): Mus Musculus (mouse)

SUBMITTER: Yoshio Kodera 

PROVIDER: PXD023475 | JPOST Repository | Sat Jan 08 00:00:00 GMT 2022

REPOSITORIES: jPOST