Identification of pomalidomide-dependent CRBN binding proteins
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ABSTRACT: The CRL4-FH-CRBN complex was purified from 293T cells stably expressing FH-CRBN and stained with CBB following SDS-PAGE. The purified complex was incubated with various cell extracts for 24 h in the presence or absence of pomalidomide. Bound proteins were then affinity-purified using anti-HA beads and subjected to SDS PAGE and CBB staining. Gel slices were prepared and subjected to MS.
ORGANISM(S): Homo Sapiens (human)
SUBMITTER: Takumi Ito and Hiroshi Handa
PROVIDER: PXD023928 | JPOST Repository | Wed Feb 02 00:00:00 GMT 2022
REPOSITORIES: jPOST
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