An efficient in-gel digestion method on a small amounts of protein sample

ABSTRACT: We have verified each step of in-gel digestion. We reduced the sample processing time and labor by optimizing the following four points. 1. Using intact gel pieces. 2. Use 96-well filter and positive pressure manifold before digestion. 3. Extract digested peptide without acetonitrile 4. In order to minimize non-specific absorption, digest in a low-peptides-binding microtube not in the 96-well filter or low-peptides-binding 96-well multiplate.

ORGANISM(S): Arabidopsis Thaliana (mouse-ear Cress)

SUBMITTER: Keiko Kuwata 

PROVIDER: PXD024071 | JPOST Repository | Thu Feb 09 00:00:00 GMT 2023

REPOSITORIES: jPOST