In-depth proteome analysis of Hela cell culture conditioned media containing fetal bovine serum.

ABSTRACT: We performed DIA-MS based proteomic analysis of Hela cells conditioned media containing fetal bovine serum by albumin (Alb)-depletion and non-depletion. In addition, we attempted to enrich secreted proteins by reducing the volume of culture medium (10mL, 5mL and 2mL). The supernatants of each culture condition were subjected to albumin depletion followed by DIA-MS. We further analyzed proteins in the conditioned media of HeLa cells with and without tumor necrosis factor (TNF) stimulation.

ORGANISM(S): Homo Sapiens (human)

SUBMITTER: Yusuke Kawashima 

PROVIDER: PXD024272 | JPOST Repository | Tue Feb 22 00:00:00 GMT 2022

REPOSITORIES: jPOST