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Proteome characterization of mesenchymal stem cells and exosomes

ABSTRACT: Proteome characterization of mesenchymal stem cells (MSC) and exosomes. MSCs were cultured in normoxic, hypoxic and in presence of FBS. Exosomes were prepared from normoxic and hypoxic conditions.

ORGANISM(S): Homo Sapiens (human)

SUBMITTER: Janne Lehtiö

PROVIDER: PXD025584 | JPOST Repository | Tue Apr 27 00:00:00 BST 2021

REPOSITORIES: jPOST

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Comprehensive Proteomic Analysis of Mesenchymal Stem Cell Exosomes Reveals Modulation of Angiogenesis via Nuclear Factor-KappaB Signaling.

Anderson Johnathon D JD Johansson Henrik J HJ Graham Calvin S CS Vesterlund Mattias M Pham Missy T MT Bramlett Charles S CS Montgomery Elizabeth N EN Mellema Matt S MS Bardini Renee L RL Contreras Zelenia Z Hoon Madeline M Bauer Gerhard G Fink Kyle D KD Fury Brian B Hendrix Kyle J KJ Chedin Frederic F El-Andaloussi Samir S Hwang Billie B Mulligan Michael S MS Lehtiö Janne J Nolta Jan A JA

Stem cells (Dayton, Ohio) 20160219 3

Mesenchymal stem cells (MSC) are known to facilitate healing of ischemic tissue related diseases through proangiogenic secretory proteins. Recent studies further show that MSC derived exosomes function as paracrine effectors of angiogenesis, however, the identity of which components of the exosome proteome responsible for this effect remains elusive. To address this we used high-resolution isoelectric focusing coupled liquid chromatography tandem mass spectrometry, an unbiased high throughput pr ...[more]

PMID: 26782178

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