Proteomics

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Facile one-pot nanoproteomics for label-free proteome profiling of 50-1000 mammalian cells

ABSTRACT: We report a facile one-pot nanoproteomics method termed SOPs-MS (Surfactant-assisted One-Pot sample processing at the standard volume coupled with MS) for convenient proteome profiling of 50-1000 mammalian cells. Building upon our recent development of SOP-MS for label-free single-cell proteomics at low µL volume (Commun Bio 2021, 4, 265), we have systematically evaluated its processing volume at 10-200 µL using 100 human cells for robust reproducible nanoproteomic analysis. The processing volume of 50 µL which is in the range of volume for standard proteomics sample preparation, has been selected for easy sample handling with benchtop micropipette. Using the commonly accessible LC-MS platform, SOPs-MS allows for reliable label-free quantification of ~1,200-2,700 protein groups from 50-1000 MCF10A cells.

ORGANISM(S): Homo Sapiens (human) Mus Musculus (mouse)

SUBMITTER: Tujin Shi 

PROVIDER: PXD026009 | JPOST Repository | Fri Jul 23 00:00:00 BST 2021

REPOSITORIES: jPOST

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Publications

Recent advances in sample preparation enable label-free mass spectrometry (MS)-based proteome profiling of small numbers of mammalian cells. However, specific devices are often required to downscale sample processing volume from the standard 50-200 μL to sub-μL for effective nanoproteomics, which greatly impedes the implementation of current nanoproteomics methods by the proteomics research community. Herein, we report a facile one-pot nanoproteomics method termed SOPs-MS (<u>s</u>urfactant-assi  ...[more]

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